Non-cultural methods for the laboratory diagnosis of invasive fungal diseases
- VernacularTitle:侵袭性真菌病的非培养实验室诊断方法
- Author:
Qiwen YANG
;
Yingchun XU
- Publication Type:Journal Article
- Keywords:
Mycoses;
Antigens,fungal;
Antibodies,fungal;
Serologic tests;
Nucleic acid amplification techniques
- From:
Chinese Journal of Laboratory Medicine
2014;(10):721-724
- CountryChina
- Language:Chinese
-
Abstract:
Non-cultural laboratory diagnostic methods include fungal antigen detection, anti-fungal antibody detection and molecular methods. Fungal antigen detection aim at ( 1, 3 )-β-D-glucan, galactomannan, cryptococcal capsular polysaccharide antigen and candida mannan antigen.Different antigen is used to diagnose different fungal infection.Antifungal antibody include antibody against Histoplasma capsulatum, Coccidioides immitis/Paracoccidiodes and Blastomyces dermatitidis.Molecular methods include non-amplification nucleic acid detection, nucleic acid amplification test, direct sequencing and matrix-assisted laser desorption/ionization time of flight mass spectrometry ( MALDI-TOF MS ) technology.Even though molecular methods are limited because of no standardization, the future of its usage is promising.
