Preparation of iRGD modified liposome and its targeting to colon cancer cell
- VernacularTitle:iRGD修饰脂质体的制备及其结肠癌靶向性研究
- Author:
Ling ZHONG
;
Siqi LI
;
Wei YANG
;
Rong ZENG
;
Qin MAO
;
Jiayong CHEN
- Publication Type:Journal Article
- Keywords:
colon cancer;
liposome;
drug targeting;
iRGD
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;37(7):69-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare iRGD modified liposome(iRGD-LP)and evaluate their targeting efficiency in vitro and in vivo to colon cancer cell.Methods The iRGD-LP was prepared by film-ultrasonic method,its particle size and Zeta potential were evaluated.The cellular uptake efficiency of RKO cell to LP and iRGD-LP were evaluated in vitro and in vivo.Tumor spheroid model were constructed and the penetration efficiency of solid tumor were evaluated.Ectopic colon cancer nude mice model was constructed,and iRGD -LP distribution in the rat body were studied.Results The particle diameter of iRGD-LP was (109.4 ±12.9)nm with the Zeta potential of (4.2 ±1.47)mV.The cellular uptake efficiency of RKO cell to iRGD-LP were 3.2 times higher than that of LP(P<0.01).The tumor spheroid penetration test and iRGD-LP distribution in vivo imaging results showed iRGD-LP had the strongest fluorescence intensity.Conclusion The iRGD-LP might serve as a promising colon cancer delivery system of antitumor drugs.
