Effect study of rapamycin on the expression of tumor helicase RECQ1 in glioma patients
- VernacularTitle:雷帕霉素对胶质瘤患者瘤体解旋酶RECQ1表达的影响研究
- Author:
Chengyong GUO
;
Zhaohui LIANG
;
Kexin WEI
;
Guozhu SUN
;
Baohua JIAO
;
Zongmao ZHAO
- Publication Type:Journal Article
- Keywords:
rapamycin;
glioma;
tumors helicase RECQ1
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;37(7):138-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of rapamycin on the expression of glioma patients tumor helicase RECQ1.Methods 50 glioma patients admitted to the department of neurosurgery in second hospital of hebei medical university were selected and randomly divided into 2 groups,25 patients in control group,were treated with routine admission surgical treatment;25 cases in the experimental group,firstly were given rapamycin capsule 1 mg,1 times/day orally,took 14 days in a row,and had surgical treatment after stopping drug a week.Glioma tissue samples were taken during the operation,mRNA and protein expression of tumor helicase RECQ1 were detected by reverse transcriptase polymerase chain reaction(RT-PCR)and Western blot.Results Glioma tumor helicase RECQ1 mRNA expression in the control group increased more significantly than experimental group,the optical density value in control group was(1.657 ±0.748),while the experimental group optical density value was(1.059 ±0.894),and the difference was statistically significant(P<0.05 );all organizations had the expression of tumor helicase RECQ1 protein,but gliomas tumor helicase RECQ1 protein expression in the experimental group patients(0.952 ±0.021)was significantly lower than that in the control group(1.211 ±0.024),and the difference was statistically significant(P<0.05).Conclusion Rapamycin capsule could reduce the expression of mRNA helicase RECQ1,inhibit DNA glial tumor cells of brain replication,effectively kill cancer cells,control the the progress of brain glioma,and improve prognosis,worth clinical promotion.
