Variations of 23S rRNA V region gene of two linezolid-intermediate En-terococcus faecalis strains
10.3969/j.issn.1671-9638.2014.10.006
- VernacularTitle:2株利奈唑胺中介粪肠球菌23S rRNA V区基因变异检测
- Author:
Jinxin ZHENG
;
Duoyun LI
;
Zhong CHEN
;
Minggui DENG
;
Xiaojun LIU
;
Qiwen DENG
;
Zhijian YU
- Publication Type:Journal Article
- Keywords:
Enterococcus faecalis;
linezolid;
drug resistance;
drug resistance,microbial;
variation;
mutation
- From:
Chinese Journal of Infection Control
2014;(10):601-604
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate antimicrobial resistance and antimicrobial resistance mechanisms of Enterococcus faecalis (E.faecalis)to linezolid (LNZ),and provide basis for clinical rational drug use.Methods Twelve E.faecalis strains isolated from sputum of patients who received LNZ therapy in a hospital between January 2012 and January 2013 were collected.The minimum inhibitory concentrations (MICs)of antimicrobial agents were de-termined by agar dilution method,23S rRNA V region gene of E.faecalis was amplified by polymerase chain reac-tion,the amplified products were sequenced.Results Of 1 2 isolates,2 were intermediate strains and 1 0 sensitive strains.The G2576U mutation was detected in 2 intermediate strains,1 of which was also detected G2424U muta-tion;the variations were not detected in 10 sensitive strains.C2424U and G2576U mutation existed in R1 and R4 region respectively.Conclusion 23S rRNA V region gene mutations are found in the intermediate strains of E.faecalis.Change in MIC values of linezolid should be paid close attention in clinical use.
