Imiquimod induces the apoptosis of THP-1 derived macrophages through TLR7 independent pathway

VernacularTitle:Imiquimod 以 TLR7非依赖途径诱导THP-1来源的巨噬细胞凋亡
Author: Xiaochen YU ; Wei YANG ; Xue GUAN ; Dan LIU ; Feng ZHOU ; Haofeng NING ; Xiuru GUAN
Publication Type:Journal Article
Keywords: Imiquimod; TLR7; Macrophage; Apoptosis
From: Chinese Journal of Microbiology and Immunology 2014;(10):759-763
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of TLR7 on imiquimod induced apoptosis of THP-1 derived macrophages.Methods Three cell lines ( THP-1 derived macrophages, MDCK cell line and HUVEC cell line) with different capabilities of expressing TLR7 were selected.The survival rates of cells af-ter the treatment with different concentrations of imiquimod were detected by MTT assay.The levels of IL-6 in the supernatants of TLR7 inhibitor chloroquine or TLR7-siRNA treated cells were detected by enzyme-linked immunosorbent assay.The apoptosis of cells was detected by flow cytometry after inhibiting the ex-pression of TLR7.Results Imiquimod induced the apoptosis of THP-1 derived macrophages, MDCK cell lines and HUVEC cell lines.The levels of IL-6 were significantly decreased as the expression of TLR7 was inhibited by treating THP-1 derived macrophages with chloroquine or TLR7-siRNA.Treating THP-1 derived macrophages with chloroquine or TLR7-siRNA did not affect the cell apoptosis induced by imiquimod.Con-clusion Imiquimod could induce the apoptosis of THP-1 derived macrophages through TLR7 independent pathway.