Analysis of the complete genome sequence of a Shenzhen coxsackievirus A2 strain SHZH13-01
10.3760/cma.j.issn.0254-5101.2014.10.008
- VernacularTitle:柯萨奇病毒 A2型深圳株 SHZH13-01全基因组序列特征分析
- Author:
Chunlin CAI
;
Xiangjie YAO
;
Fei ZHUO
;
Yaqing HE
;
Guiqing YANG
- Publication Type:Journal Article
- Keywords:
Coxsackievirus A2;
Complete genome;
Sequence analysis
- From:
Chinese Journal of Microbiology and Immunology
2014;(10):770-773
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the complete genome sequence of a Shenzhen coxsackievirus A2 strain CVA2-SHZH13-01 and its evolution.Methods RT-PCR was used to amplify the complete genome of CVA2-SHZH13-01 strain.The PCR products were purified and sequenced to analyze their genetic character-istics.Results The complete genome of CVA2-SHZH13-01 strain was 7400 bp in length, encoding 2191 amino acids.CVA2-SHZH13-01 strain was highly similar with the novel recombinant CVA2-HK (431306) strain isolated from Hong Kong sharing the nucleotide homology of 98.3%, 98.8%, 99.0%, 99.2%, 98.8%and 98.9%in 5′UTR, P1 ( VP1 to VP4) , P2, P3, 3′UTR regions and complete genome, respec-tively.CVA2-SHZH13-01 strain was highly identical to the international standard strain CVA2-Fleetwood showing the homology of 81.6% in nucleotide sequences in P1 region, but closely associated with EV71-SHZH03 and EV71-GD2009 strains (82.8%-88.7%) in P2 and P3 regions.The phylogenetic analysis in-dicated that CVA2-SHZH13-01 strain belonged to the CVA2-HK (431306) variant.Data from analysis of amino acid in P1 region showed that there were three amino acid mutations in CVA2-SHZH13-01 strain including aa5L→F, aa666S→G and aa671T→I as compared with CVA2-HK (431306) strain.Conclusion CVA2-SHZH13-01 strain belonged to CVA2-HK (431306) variant.
