Roles and mechanisms of Fas/FasL in the apoptosis of HL-60 cells induced by euphorbiasteroid
10.3760/cma.j.issn.1673-422X.2014.09.012
- VernacularTitle:Fas／FasL信号通路在千金子甾醇诱导HL-60细胞凋亡中的作用与机制研究
- Author:
Fei GUO
;
Xia LI
;
Chao ZHANG
;
Xia REN
;
Meiyan SHI
;
Guosheng JIANG
- Publication Type:Journal Article
- Keywords:
HL-60 cells;
Apoptosis;
Fas/FasL;
Euphorbiasteroid
- From:
Journal of International Oncology
2014;(9):679-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of euphorbiasteroid on inducing the apoptosis of HL-60 cells and demonstrate whether the Fas/FasL signaling pathway is involved in the induction of apoptosis. Methods HL-60 cells were treated with dose of 2.5,10,40 μg/ml of euphorbiasteroid in vitro for 24 h respectively.After that,cell counting Kit-8 was used to detect cell proliferation.The morphology of HL-60 cells were observed under light and fluorescent microscopy.The early cell apoptosis was detected by using flow cytometry with Annexin Ⅴ-FITC /PI double staining.The expressions of Fas,FasL,caspase-8 and caspase-3 mRNA were analyzed by the method of RT-PCR.The activities of caspase-8 and caspase-3 were examined by chromatometry.Results Compared with 1640 control group,HL-60 cell proliferation was inhibited significant-ly by euphorbiasteroid.The inhibition rates were (34.9 ±3.7)%,(54.6 ±5.2)% and (61.3 ±4.3)%respectively.Moreover,HL-60 cells exhibited typical morphological features.Early cell apoptosis rates of HL-60 cells were (23.4 ±3.1)%,(35.7 ±4.3)% and (53.2 ±3.9)% respectively.Furthermore,the expressions of Fas,FasL,caspase-3 and caspase-8 mRNA were up-regulated significantly after euphorbiasteroid administration in a dose-dependent manner (P<0.01 ).After treated with euphorbiasteroid,the activities of caspase-8 and caspase-3 were significantly enhanced (P<0.01 ).Conclusion The up-regulation effect of euphorbiasteroid on Fas/FasL signaling pathway might contribute to the apoptosis of HL-60 cells.
