Protective effect of peperphentonamine hydrochloride on the gentamicin-induced cochlear afferent nerve system damage of guinea pigs
10.3969/j.issn.1006-5725.2014.19.004
- VernacularTitle:盐酸椒苯酮胺对庆大霉素致豚鼠耳蜗传入神经损伤的保护作用
- Author:
Wei LI
;
Hao CHEN
;
Jian WU
;
Yonghe LI
- Publication Type:Journal Article
- Keywords:
Peperphentonamine hydrochloride;
Gentamicin;
Guinea pigs;
Afferent nerve system damage;
Apoptosis
- From:
The Journal of Practical Medicine
2014;(19):3045-3048
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of peperphentonamine hydrochloride (PPTA) on the cochlea afferent nerve system damage induced by gentamicin (GM) of guinea pigs. Methods Thirty-six health guinea pigs were randomly divided into three groups:the normal group with saline; GM group with muscle injection of GM [100 mg/(kg·d),14 d] and PPTA group with peritoneal injection of PPTA [10 mg/(kg·d),14 d] on the ABR was analyzed , and the expression of Caspase-3 was tested by Western blot. TUNEL (after coloration)was performed to observe the apoptotic index,and its morphological changes were observed by TEM. Results After trial, the threshold of control group, GM group, PPTA group were 14.58 ± 1.16, 65.95 ± 1.17, 36.13 ± 1.17;the apoptosis index: 1.09 ± 0.14, 23.17 ± 0.88, 8.84 ± 0.49; the Caspase-3:1.09 ± 0.11, 2.55 ± 0.20, 1.67 ± 0.07. There were significantly difference among three groups (P<0.05). Morphological changes: cells apoptosis and the synapase were damaged seriously in GM group; the injury was less serious in PPTA group. Conclusion PPTA plays a protective role on GM-induced cochlea afferent nerve system damage of guinea pigs.
