Content Determination of Dryocrassine in Guanye Jinsitao Drop Pills by HPLC

VernacularTitle:HPLC测定贯叶金丝桃滴丸中绵马贯众素含量
Author: Peng ZHOU ; Guangshang CAO
Publication Type:Journal Article
Keywords: Guanye Jinsitao drop pills; dryocrassine; HPLC
From: Chinese Journal of Information on Traditional Chinese Medicine 2014;(10):89-91
CountryChina
Language:Chinese
Abstract: Objective To develop an assay of HPLC for the content determination of dryocrassine in Guanye Jinsitao drop pills. Methods The column was Kromasil-C18 (150 mm×4.6 mm, 5μm);mobile phase was acetonitrile-chloroform-isopropanol-0.1% phosphoric acid (52∶8∶30∶10);flow rate was 1.0 mL/min;UV detection was performed at 285 nm;detected drift tube temperature was set at 25 ℃. Results The calibration curves were linear in the range of 0.236-2.124 μg for dryocrassine (r=0.999 9). The average recovery rate of dryocrassine was 99.64% (RSD=1.90%). Conclusion The method is simple and accurate. It is suitable for the determination of dryocrassine in Guanye Jinsitao drop pills.