Impact of NBP on expression of HSP70 and TLR4 in rat with ischemia-reperfu-sion

VernacularTitle:丁苯酞对脑缺血再灌注大鼠HSP70及TLR4的影响研究
Author: Xiaoxuan ZHANG ; Haipeng QIU ; Shumin ZHAO
Publication Type:Journal Article
Keywords: NBP; Ischemia-reperfusion; HSP70; TLR4
From: Chinese Journal of Immunology 2014;(10):1401-1404
CountryChina
Language:Chinese
Abstract: Objective:To analyze the impact of NBP on the expression of HSP 70 and TLR4 in the rat with ischemia-reperfusion.Methods:The 144 rats were divided into 3 groups,Sham group,IR group and NBP group.The 3 groups were divided into 4 groups again according to the time of reperfusion (6h,12h,24h and 48h).The intraluminal ischemia-reperfusion model was made by improved Longa.When the model was made successfully ,the rat in NBP group was gave oral NBP immediately while the IR and Sham group was gave oral NS.The neurological deficit scores , number of apoptotic cells , expression of HSP70 and TLR4 were compared during the 3 groups.Results:The neurological function ,apoptosis,the expression of HSP70 and TLR4 in NBP group at any reperfusion time were statistical lower than in IR group.In all the infusion periods,apoptotic cells in IR and NBP group were significantly higher than that in the Sham group.In all the infusion periods ,the positive cells in IR and NBP group were significantly lower than that in the Sham group.Conclusion:NBP can protect the function of neuron by improving the inhibition of HSP 70 and TLR4.