Effect of oleanolic acid derivatives on improving insulin resistance and its molecular mechanism
10.3969/j.issn.1001-1978.2014.11.021
- VernacularTitle:齐墩果酸衍生物对胰岛素抵抗的改善作用及机制
- Author:
Jing XU
;
Linhui ZHU
;
Debin WANG
;
Xin HU
;
Guangzhong YANG
- Publication Type:Journal Article
- Keywords:
oleanolic acid;
Bio;
diabetes;
insulin re-sistance;
HepG2 cells;
PPARγ
- From:
Chinese Pharmacological Bulletin
2014;(11):1585-1589
- CountryChina
- Language:Chinese
-
Abstract:
Aim To detect the effect of Bio on impro-ving insulin resistance and explore its molecular mech-anism. Methods The HepG2 liver cells were derivat-ed by high concentration insulin to establish the insulin resistance cell model, and the cells were intervened by Bio. The glucose consumption was measured by glu-cose oxidase and peroxidase ( GOD-POD) assay. The expression of PPARγmRNA was detected by RT-PCR. The expression of PPARγ protein was detected by Western blot method. Results The glucose consump-tion was significantly decreased in the insulin resist-ance cells after incubated with 1 . 72 × 10 -5 mol · L-1 insulin ( P<0. 05 ) . 10 -5 ,10 -6 ,10 -7 mol · L-1 Bio increased the glucose consumption 135%,62%,39%separately in the insulin resistance cells. RT-PCR a-nalysis of PPARγ showed that Bio raised the PPARγmRNA. Western blot analysis displayed that the pro-tein of PPARγ with Bio was increased. Conclusion Bio can improve the insulin resistance of the HepG2 cells, and the molecular mechanism may be relevant with raising PPARγ expression.
