Preparation and immunogenicity and specificity characterization of Emodin-BSA-PVDF membrane artificial antigen
10.3969/j.issn.1000-484X.2015.01.019
- VernacularTitle:大黄素－BSA包被膜片人工抗原制备及其免疫原性与特异性检测
- Author:
Lihua LI
;
Wentai LIU
;
Huazhou XU
;
Guoping CHANG
;
Nan WANG
;
Yuxin TIAN
;
Xuexin SONG
;
Ziye ZHANG
- Publication Type:Journal Article
- Keywords:
Hapten;
Emodin;
Artificial antigen;
Membrane coated;
Subcutaneous implantation
- From:
Chinese Journal of Immunology
2015;(1):90-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study feasibility of preparing artificial antigen by membrane coated with hapten-carrier.To compare the Emodin-BSA membrane antigen immunogenicity and specificity against the liquid antigen.Methods:Emodin-BSA-PVDF membrane was prepared by the method that BSA was coated on PVDF membrane and the BSA was coupled with Emodin-couplint agent derivative.Rats were immunized by subcutaneous implantation.The immunogenicity and antibody specificity were characterized using Emodin-CA or Chrysophanol-CA or Physcion-CA membrane immunoassay. Results: The immunogenicity of Emodin-BSA coated membrane antigen was higher than Emodin-BSA liquid antigen;the specificity for three anthraquinones was almost the same(P>0.05). Conclusion:Emodin antiserum generated using Emodin-BSA coated membrane antigen has a high immunogenicity and specificity to Emodin.The results show it is feasible that membrane coated with hapten-carrier is used as artificial antigen.
