Surveillance and forecast system of schistosomiasis in Jiangsu Province ⅥDetection technology of water infectivity based on enrichment of Schistosoma japonicum cercariae on water surface
- VernacularTitle:江苏省血吸虫病监测预警系统的研究Ⅵ基于水面日本血吸虫尾蚴富集的水体感染性检测技术
- Author:
Guoli QU
;
Jianrong DAI
;
Yuantian XING
;
Wei WANG
;
Zhenkun YANG
;
Zhengyang ZHAO
;
Na GUO
;
Leping SUN
;
Yousheng LIANG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Forecast;
Surveillance;
Expanding agents;
C-6 film;
Cercaria
- From:
Chinese Journal of Schistosomiasis Control
2014;(5):510-513
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the enrichment technique of Schistosoma japonicum cercariae on the water surface,so as to establish a new method combined with the existing technology to detect the cercarial infested water body quickly and sensitive-ly. Methods Soybean oil,gasoline,kerosene and isophorone were screened as expanding agents. The cercariae were enriched by the thrust of the expanding agents when diffusing on the water surface,and PE adsorption film and C-6 film were applied to seize them so as to determine the infectivity of the water quickly. The relationship between the dose of expanding agents and dif-fusion radius were explored. Results Gasoline,kerosene and isophorone were suitable expanding agents,and the diffusion ef-fect of isophorone was the best. After the enrichment by the expanding agents,the detection rate of cercariae of the method seiz-ing cercariae with the film significantly improved in the water. Conclusion This new method could effectively improve the de-tection rate of the cercarial infested water and is suitable for the low-degree infested water.
