Analysis of the function of the UBE gene based on bioinformatics
- VernacularTitle:泛素结合酶基因生物信息学与功能分析
- Author:
Yang YAO
;
Jie SU
;
Di WEI
;
Xingmei ZHU
;
Biping CHENG
- Publication Type:Journal Article
- Keywords:
HBV-related hepatocellular carcinoma;
Bioinformatics;
Ubiquitin-conjugating enzyme 2S
- From:
Journal of Medical Postgraduates
2014;(11):1156-1159
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract ] Objective Bioinformatics provides a lot of valuable information for online prediction of new genes.In this study, we predicted the biological function of ubiquitin-conjugating enzyme 2S ( UBE2S) based on bioinformatics. Methods The UBE2S gene was screened and cloned from the cDNA library of human HepG2 cells.The relationship of the structure and function of UBE2S was explored based on the full-length cDNA library.MEGA5.05, CLUSTALW2 and SWISS-MODEL were used to study the phylogeny, conservation, and 3D structure of UBE2S. Results The UBE2S gene encoded a polypeptide of 241 residues with a predicted molec-ular weight of 23 770 and an isoelectric point of 8.81.The UBE2S protein contained no transmembrane locus and the probabilities of their functions of growth factors, cation channel and structural protein were 8.904, 0.313, and 0.291.The analysis of BLASTp showed that the isolated UBE2S had a 90-97%identity with the other species. Conclusion Analysis of the structure and function of the UBE2S protein can not only provide more information about its gene family but also pave the way for further experimental studies on the molecular mechanism of the consequent hepatocellular carcinoma.
