Adjuvant effect of CTA1-DD/IgG on immune responses to mode antigen ovalbu-min
10.3969/j.issn.1000-484X.2014.10.011
- VernacularTitle:用模式抗原OVA研究CTA1-DD／IgG佐剂的免疫调节作用
- Author:
Wenjiong XU
;
Yan WANG
;
Xuan WANG
;
Mengkai QIAO
;
Min DONG
;
Min HE
;
Limin SHI
;
Xiaoxiao DONG
;
Shifeng YUN
;
Jie DING
- Publication Type:Journal Article
- Keywords:
OVA;
Adjuvant;
Immunoregulation;
CTA1-DD
- From:
Chinese Journal of Immunology
2014;(10):1349-1352
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the immunological effects of a new type adjuvant CTA 1-DD/IgG.Methods:Intranasal im-munization of BALB/c mice with OVA in combination with adjuvant CTA 1-DD or CTA1-DD/IgG.Blood samples were subjected to OVA-specific IgG Abs detection and IgG subclass profiles assessment using ELISA.Splenic plasma cells secreting anti-OVA Abs were detected by ELISPOT.Results:CTA1-DD/IgG showed stronger activity in inducing OVA-specific IgG Abs and splenic Abs-secreting cells compared with CTA1-DD.The enhancement of IgG1,IgG2a and IgG2b Abs were observed,and the ratio IgG2a/IgG1 were >1 in both groups.Conclusion: CTA1-DD/IgG can exert enhanced adjuvant effects compared with CTA 1-DD.Mixed IgG subclasses are induced,indicating a more balanced Th1/Th2 response.
