Effects of Dehydrocorydaline on Proliferation and Collagen Secretion of Cardiac Fibroblasts Cultured by High Glucose
10.3969/j.issn.1005-5304.2014.10.015
- VernacularTitle:脱氢紫堇碱对高糖培养心肌成纤维细胞增殖和胶原分泌的影响
- Author:
Lutuo HAN
;
Jiamei YANG
;
Junguo REN
;
Honghai LI
;
Junmei LI
;
Jianxun LIU
- Publication Type:Journal Article
- Keywords:
dehydrocorydaline;
cultured in vitro with high glucose;
cardiac fibroblasts;
cell proliferation;
collagen
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(10):50-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of dehydrocorydaline (DHC) on proliferation and collagen secretion of cardiac fibroblasts (CFs) cultured by high glucose;To provide experimental evidence for clinical application of Rhizoma Corydalis. Methods CFs cultured in vitro with high glucose were made into models. Collagenase and trypsin were used for the combine digestion of CFs from ratsbornin 24 h. 2-4 generation CFs were cultured by high glucose (25 mmol/L), and then 100, 50, 25 mg/L dethydrocorydaline was added for intervention. Cellular morphology of CFs was observed after 24, 48 h. CFs proliferation was detected by MTT method. Cell cycle was assessed via flow cytometry. The levels of collagen Ⅰ and collagen Ⅲ were determined by ELISA. Results CFs began to grow adherence 3 hours after planting, and CFs cultured by high glucose significantly proliferated 24, 48 h later (P<0.05, P<0.01). The percentage of S+G2+M phase CFs increased significantly after 48 h (P<0.01). The secretion of collagen Ⅰ and collagen Ⅲ also increased significantly (P<0.01). After the intervention of DHC, CFs proliferation was significantly inhibited (P<0.01);the percentage of S+G2+M phase CFs decreased (P<0.01);the secretion of collagen Ⅰ and collagen Ⅲ was reduced (P<0.05, P<0.01). Conclusion DHC can reduce CFs proliferation, decrease collagen secretion of CFs cultured by high glucose, and has potential effects of anti-myocardial fibrosis.
