Effect of Paecilomyces hepiali mycelium on physical fatigue and hypoxia in mice
10.7644/j.issn.1674-9960.2014.10.010
- VernacularTitle:蝙蝠蛾拟青霉菌丝体对小鼠体力疲劳及缺氧的影响
- Author:
Yuhui XU
;
Huichun LIANG
;
Quan WEN
;
Enmao YE
;
Zheng YANG
- Publication Type:Journal Article
- Keywords:
Paecilomyces hepiali;
Cordyceps;
fatigue;
anoxia
- From:
Military Medical Sciences
2014;(10):784-790
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-fatigue and anti-hypoxia effect of fermented Paecilomyces hepiali myceli-um (PHM) and to explore its mechanism.Methods Seventy-two naive Kunming male mice were randomly divided into Home, vehicle, modafinil( Mode) , low-dose PHM, moderate-dose PHM, and high-dose PHM groups.All mice were ad-ministrated with the drug or vehicle twice a day during a 14-day period.Except for the Home group, each groups was forced into the wheel fatigue device to receive a climb-run training at the time points of 40 min, 8 h, 24 h and 48 h, and to re-ceive an exhaustive training at the time point of 72 h after the last administration.The number of electric shocks was recor-ded during each training and the levels of muscle glycogen, liver glycogen, serum urea nitrogen, serum lactic acid, serum creatine kinase and blood ATP were detected after the exhaustive training using commercially available kits.In addition, 96 naive Kunming male mice were randomly and equally divided into the vehicle, low-dose, moderate-dose and high-dose PHM groups.Twelve mice in each group were chosen for the normal pressure anti-hypoxia experiment and the other 12 mice were used for the NaNO3 poisoning experiment.Results Compared with the vehicle groups, the PHM group showed a sig-nificant decrease in the number of electric shocks, improvement in biochemical parameters associated with fatigue, and an increased survival time in the anti-hypoxia and the NaNO3 toxicity tests.Conclusion PHM is potentially an effective alter-native for wild Cordyceps in the treatment of fatigue and hypoxia.
