Determination of contents and related substances of demethyl levophencynonate hydrochloride tablets by HPLC
10.7644/j.issn.1674-9960.2014.10.016
- VernacularTitle:HPLC法测定盐酸左旋去甲基苯环壬酯片的含量及有关物质
- Author:
Jianxu SUN
;
Xiaoqing WANG
;
Xiaoyan ZHANG
;
Jiaojiao LI
;
Bohua ZHONG
;
Jianquan ZHENG
;
Xinhua HE
;
Liyun WANG
;
Aiping ZHENG
- Publication Type:Journal Article
- Keywords:
demethyl levophencynonate hydrochloride tablets;
HPLC;
contents;
related substance
- From:
Military Medical Sciences
2014;(10):811-813
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple, feasible and precise quality control method for the determination of contents and related substances of demethyl levophencynonate hydrochloride (L-LPC)tablets.Methods The mobile phase consisted of methanol,acetonitrile and sodium acetate buffer solution(pH 5.0),at a ratio of 4∶3∶3,at a flow rate 1.0 ml/min and a detection wavelength of 220 nm.Samples were injected 100 μl and determined at room temperature.Results The calibration curves showed good linearity (R2 =1) within the test range of 0.1-50μg/ml.The recovery of the method was about (100.15 ±0.73)%, and the stability of working solutions was acceptable in 8 h (RSD=0.36%).Conclusion The results indicated that the developed method can be readily used as a quality control method.
