Effect of Radix Isatidis polysaccharide on nitric oxide and endothelin-1 after ortho-topic liver autotransplantation in rats
10.7644/j.issn.1674-9960.2014.10.017
- VernacularTitle:板蓝根多糖对大鼠自体肝移植后一氧化氮和内皮素-1的影响
- Author:
Jie LANG
;
Peijian ZHANG
- Publication Type:Journal Article
- Keywords:
Radix Isatidis polysaccharide;
orthotopic liver autotransplantation;
hepatic ischemia reperfusion injury;
ni-tric oxide;
endothelin-1
- From:Military Medical Sciences
2014;(10):814-818
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Radix Isatidis( indigowoad root,RI) polysaccharide on NO and ET-1 in orthopotic liver autotransplantation of rats.Methods The rats used in the experiment were randomly divided into three groups:normal control group (NC), autotransplantation group (AT) and RI polysaccharide group (RIPS).At 1, 6, 12 and 24 hours after autotransplantation, the content of NO,ET-1, alanine aminotransferase ( ALT), and aspartate amin-otransferase ( AST) in serum was assayed.Morphological observation of hepatic tissues was also performed.Results The serum level of ET-1 in RIPS group was significantly lower than that of AT group (P<0.05), and NO content in serum of RIPS group was increased significantly compared with AT group ( P<0.05) at 1, 6, 12 and 24 hours after autotransplanta-tion.The serum level of ALT and AST in RIPS group was lower than that of AT group (P<0.05) at each measurement point.The morphology of hepatocytes changed abnormally under light microscopy.The blood stasis and swelling of hepatic tissues, denaturalization and necrosis of hepatocytes in RIPS group were milder than that in AT group.Conclusion RIPS has a protective effect on hepatic ischemia reperfusion injury after orthotopic liver autotransplantation, which may be related to increased NO levels and lower levels of ET-1.
