Preparation and evaluation of insulin thiolated hyaluronic acid nanoparticles in vitro
- VernacularTitle:胰岛素巯基化透明质酸纳米粒的制备与体外性质评价
- Author:
Aiwen HUANG
;
Jiali ZHAO
;
Zhihong LIU
;
Qiping ZENG
;
Hongtao SONG
- Publication Type:Journal Article
- Keywords:
thiolated hyaluronic acid;
insulin;
nanoparticles
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;37(7):77-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare insulin thiolated hyaluronic acid nanoparticles (Ins-HA-Cys-NPs)and study its physicochemical properties. Methods The Ins-HA-Cys-NPs was prepared by ultrasonic emulsifying method,and the properties of nanoparticles including morphology,mean diameter,Zeta potential,entrapment efficiency and drug loading efficiency were studied,as well as the cryoprotectant selection.Results The prepared nanoparticles was round in appearance and the mean diameter was(178.5 ±0.8)nm,the polydispersity index was (0.214 ±0.013)and the Zeta potential was -(38.47 ±0.46 )mV,while the entrapment efficiency was (48.85 ±0.66 )%,drug loading efficiency was (4.79 ±0.13 )%;10%mannitol as cryoprotectant provided uniform and well dispersed suspension of nanoparticles with blue opalescence after redispersion.Conclusion The thiolated hyaluronic acid nanoparticles may be used as the carrier for oral drug delivery system of insulin,and it provides a basis for studies on rats in vivo.
