Effect of GABA on expression of VEGF and MMP-9 in monocrotaline-induced pulmonary hypertension rats

VernacularTitle:γ氨基丁酸对肺动脉高压大鼠肺组织VEGF、MMP-9表达的影响
Author: Gang CHEN ; Jun SONG ; Hong XIONG ; Lujie ZHANG ; Jinquan LV
Publication Type:Journal Article
Keywords: hypertension,pulmonary; γ-aminobutyric(GABA); VEGF; MMP-9; monocrotaline
From: Chongqing Medicine 2014;(30):4043-4045
CountryChina
Language:Chinese
Abstract: Objective To study the therapeutical effect of γ-aminobutyric acid on monocrotaline(MCT )induced pulmonary hy-pertension rats ,and to elucidate the expression of VEGF and MMP-9 .Methods Thirty SD rats were randomly divided into 3 groups :a normal control group(control group) ,a MCT-induced pulmonary hypertension group(model control group) ,and anγ-ami-nobutyric acid treatment group(treatment group) .The mean right ventricular pressure(mRVP)were detected ,the right ventricular hypertrophy index(RVHI)were measured ,WT% and WA% were evaluated ,and the expression of VEGF mRNA in the lung tissue and MMP-9 were detected wtih FQ-PCR and immunohistochemical staining method respectively .Results mRVP ,RVHl ,WT% , WA% and the expression of MMP-9 and VEGF mRNA of treatment group were lower than those in the model control group(P<0 .05) ,but higher than the control group(P<0 .05) .Conclusion GABA has a therapeutic effect on pulmonary hypertension rats through regulating the expression of VEGF mRNA and MMP-9 protein .