Determination of Trace Gibberellin A3 by Magnetic Self-assembly Molecularly Imprinted Electrochemical Sensor
10.11895/j.issn.0253-3820.140552
- VernacularTitle:基于磁性自组装分子印迹传感器测定赤霉素A3
- Author:
Lianming ZHANG
;
Xiaoping WEI
;
Yanxi WEI
;
Jianping LI
;
Ying ZENG
- Publication Type:Journal Article
- Keywords:
Molecular imprinted;
Gibberellins A3;
Self-assembly;
Magnetic nanoparticle
- From:
Chinese Journal of Analytical Chemistry
2014;(11):1580-1585
- CountryChina
- Language:Chinese
-
Abstract:
ThemagneticFe3O4nanoparticlesweresynthesizedbyco-precipitationmethod,andthenmagnetic Fe3 O4@Au nanoparticle was synthesized to improve the affinity of particle surface. L-Cys-GA3 was grafted on the surface of gold clad by self-assembly method, and then dropped it on glassy carbon electrode, for further manufacture of MIP/Fe3 O4@Au by using electropolymerzation L-Cys. The surface morphology and particle size distribution of Fe3 O4@Au were studied by TEM. The structure and composition of gibberellins A3, MIP and nMIP were studied by IR. The test system was optimized, and the results showed that when the cycles of electropolymerization was 30, acetic acid:methanol (1:8, V/V) was chosen as eluent, elution time was optimized for 5 min and rebinding time for 7 min, the sensor got a high stability and good recognition ability for gibberellins A3 . The concentration of gibberellins A3 in the range of 1 . 0 × 10-11-1 . 0 × 10-8 mol/L had a relationship with the oxidation peak current of probe, with the detection limit of 2. 57×10-12 mol/L. The sensor was successfully used for the determination of GA3 in beer sample.
