Quantum Dots Labeled Lateral Flow Strip for Determination of Procalcitonin in Blood
10.11895/j.issn.0253-3820.140453
- VernacularTitle:量子点标记免疫层析技术检测血液中的降钙素原
- Author:
Hao YU
;
Liang XU
;
Xiaoping QI
- Publication Type:Journal Article
- Keywords:
Quantum dot;
Immunochromatography;
Procalcitonin;
Blood;
Lateral flow strip
- From:
Chinese Journal of Analytical Chemistry
2014;(11):1592-1597
- CountryChina
- Language:Chinese
-
Abstract:
CdSe/ZnSquantumdots(QDs)werepreparedandcovalentlylinkedtoanti-katacalcinmonoclonal antibodies. After modification, the QDs' maximum emission wavelength was shifted to 625 nm from 620 nm while maintaining the spectral properties. Then the QDs labeled lateral flow strip and corresponding fluorescence measuring instrument were designed and fabricated. To reduce the cost of strip by reducing the amounts of monoclonal antibodies, appropriate amounts of QDs labeled monoclonal antibodies were sprayed on the conjugation pad, with just one test line on the strip but without the control line. Parameters of the strip were optimized by measuring the signal to noise ratio. By using the strip and fluorescence measuring instrument, procalcitonin (PCT) could be detected in 20 min, and the quantitative detection range was 0. 2-100 μg/L with sensitivity of 0. 1 μg/L. A total of 22 blood samples were measured by both our method and the commercial instrument used in the hoptital. The results were consistent for their Pearson correlation coefficient (0. 9995) and Kolmogorov-Smirnov test (Sig=1. 0). The rapid quantitative detection method for PCT is of great importance to quantitative detection of bacterial infection and rational usage of antibiotics clinically.
