Preparation, Characterization and Application for Genomic DNA Extraction of a Magnetic Microsphere with Surface Charge Switching
10.11895/j.issn.0253-3820.140434
- VernacularTitle:表面电性可控的磁性微球的制备、表征及其在基因组DNA萃取中的应用
- Author:
Huabin HUANG
;
Zhixia ZHUANG
;
Chaoyong YANG
;
Xiaoru WANG
- Publication Type:Journal Article
- Keywords:
Surface charge switching;
Magnetic microsphere;
Deoxyribonucleic acid extraction
- From:
Chinese Journal of Analytical Chemistry
2014;(11):1598-1603
- CountryChina
- Language:Chinese
-
Abstract:
SuperparamagneticSiO2@Fe3O4microspherewithcore-shellstructurewaspreparedbythe method of hydro-thermal and St?ber method, and modified by (3-aminopropyl) triethoxysilane ( APTES) . The obtained amino-Fe3 O4@SiO2 microsphere with controlled surface charge properties was characterized by SEM and TEM, and the surface electric property of amino-microsphere was investigated by Zeta potential measurement. The microsphere was used to extract DNA from human blood, and the solid phase extraction method by microspheres was developed. The mechanism of action between Aminated-Fe3 O4@SiO2 microsphere and DNA was explored, and the gel electrophoresis and PCR test were done on the extraction product. The result showed that genomic DNA with high purity was successfully extracted from whole blood by using amino-Fe3 O4@SiO2 microsphere, with the extraction rate about 70%, the saturated adsorption capacity for DNA was about 40 ng/μg, and the elution could be directly used for further bio-analysis.
