Investigation of mechanism of SchA to decreasing MPP+-induced SH-SY5Y cell damage
10.3969/j.issn.1671-8348.2014.29.029
- VernacularTitle:五味子甲素SchA缓解MPP＋诱导的SH-SY5Y细胞损伤的机制研究
- Author:
Pengyan JI
;
Yan LI
;
Wei ZHANG
;
Shunli PENG
;
Zhe HE
- Publication Type:Journal Article
- Keywords:
schizandrin;
MMP+;
SH-SY5Y cell;
protein kinases
- From:
Chongqing Medicine
2014;(29):3932-3934
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the possible mechanism of SchA ,which decreases MPP+induce SH-SY5Y cell damage .Meth-ods Cultured cells were divided into 5 groups ,one as control group ,cultured by free-blood serum media;the other 4 groups were treated with different concentrations of SchA(1 ,3 ,5 μmol/L) and MPP+ (1 mmol/L) for 48 h named model group ,1 ,3 ,5 μmol/L SchA group respetivly .The content of nitric oxide(NO) were measured by NO kit ;The expression levels of total Akt and p-Akt proteins were detected by Western blot .Results Compared with the control group ,the content of NO in group significantly in-creased after MPP+stimulating(P<0 .05);compared to the control group ,the content of NO in 5μmol/L SchA group significantly decreased(P<0 .05) .The expression levels of total Akt in all groups had no significant difference(P>0 .05) .The expression levels of p-Akt in model group significantly lowered ,while SchA(1、3、5 μmol/L) significantly increased the expression levels of p-Akt in comparision with cells in model group .Conclusion Decreasing MPP+ induced SH-SY5Y cell damage of SchA may be related to the content of NO and p-Akt expression .
