Effect of Ghrelin on L-type Calcium Channel Current of Ventricular Myocytes in Experimental Rats
10.3969/j.issn.1000-3614.2014.10.018
- VernacularTitle:Ghrelin对大鼠心室肌细胞L型钙电流的影响
- Author:
Bo YANG
;
Daoliang ZHANG
;
Youzhi HU
;
Jinfeng XIAO
;
Chengzhi ZHOU
- Publication Type:Journal Article
- Keywords:
Ghrelin;
L-type calcium channel;
Whole-cell patch-clamp
- From:
Chinese Circulation Journal
2014;(10):832-835
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of ghrelin on L-type calcium channel current (ICa-L) of ventricular myocytes in experimental rats. Methods: The single ventricular myocyte in experimental rats were obtained by enzymolysis method, and the whole-cell patch-clamp technique was used to investigate the effect of ghrelin on ICa-L of ventricular myocytes at different doses of 10 nmol/L, 100 nmol/L and 1μmol/L respectively. Results: Ghrelin at 10 nmol/L, 100 nmol/L and 1μmol/L may inhibit ICa-L at (8.95 ± 2.13) %, (31.18 ± 4.78) % and (64.63 ± 8.57)% respectively,P<0.05, and the current-voltage curve was shifted upwards. The channel half inactivation curve decreased from (-1.34 ± 1.9) mV to (-8.04 ± 1.32 ) mV, (9.76 ± 1.17) mV and (-11.81 ± 0.73) mV respectively,P<0.05, and the recovery time after inactivation was prolonged as τ value from (63.23 ± 9.32) to (98.95 ± 10.74), (109.56 ± 13.42) and (127.39 ± 16.13) respectively,P<0.05. Conclusion: Ghrelin may accelerate ICa-L inactivation and prolong the recovery time after inactivation. Ghrelin inhibits ICa-L in a dose-dependent manner.
