Study on the mechanism of Arsenic Trioxide inducing the apoptosis of myelodysplastic syndrome cell line SKM-1
10.3969/j.issn.1671-8348.2014.29.017
- VernacularTitle:三氧化二砷诱导骨髓增生异常综合征SKM-1细胞凋亡机制的研究
- Author:
Haiying HUA
;
Huaqiang GAO
;
Aining SUN
;
Wenyan ZHU
;
Jiannong CEN
;
Lili WU
;
Donglin JIANG
- Publication Type:Journal Article
- Keywords:
myelodysplastic syndromes;
AS2 O3;
apoptosis;
Bcl-2;
Bax;
caspase-3
- From:
Chongqing Medicine
2014;(29):3897-3900
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of AS2 O3 inducing the apoptosis of myelodysplastic syndrome(MDS) cell line SKM-1 .Methods SKM-1 cells were incubated with AS2 O3 ,and then the cellular morphology was observed ,flow cytometry was used to determine the apoptosis ,RT-PCR was used to detect the expressions of Bcl-2 ,Bax and caspase-3 mRNA .Results 0 .25、0 .50 μmol/L AS2 O3 could not markedly induce the apoptosis of SKM-1 cells (P>0 .05) .But 2 .00、8 .00、32 .00 μmol/L of AS2 O3 could obviously promote the apoptosis of SKM-1 cells .With the increase of the acting time and concentration of AS2 O3 ,the apoptosis rate increased ,too(P<0 .01) ,the expressions of anti-apoptotic gene Bcl-2 mRNA decreased (P<0 .01) ,the expressions of promoting apoptosis gene Bax and caspase-3 mRNA increased (P<0 .01 ,P<0 .05) .Conclusion 2 .00、8 .00、32 .00 μmol/L of AS2O3 may promote the apoptosis of SKM-1 cells through down-regulating the expression of Bcl-2 gene and up-regulating the ex-pressions of Bax and caspase-3 genes .
