Research on peripheral T, B cell subsets and NK cells under different immune status and hepatitis B cirrhosis with chronic HBV infection
10.3969/j.issn.1006-5725.2014.20.010
- VernacularTitle:慢性HBV感染不同免疫状态及乙肝肝硬化外周血T、B细胞亚群和NK细胞的变化及意义
- Author:
Min ZHANG
;
Lisha YANG
;
Dezhen PENG
;
Bing ZHANG
;
Lin NIE
- Publication Type:Journal Article
- Keywords:
T-lymphocytes;
B-lymphocytes;
Killer cells;
Chronic hepatitis B;
Liver cirrhosis
- From:
The Journal of Practical Medicine
2014;(20):3233-3236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the percentage changes of peripheral T , B cell subsets and NK cells in chronic HBV infectors under different immune states and hepatitis B cirrhosis . Methods Seventy-five chronic HBV infectors, including 20 cases with immune clearance, 20 cases with immunodeficiency (inactive) and 35 cases with cirrhosis, and 20 healthy control were enrolled. The percentages of peripheral T and B lymphocyte subsets and NK cells were detected by Flow Cytometry. The differences of the groups were analyzed. Results Comparing with the control group, CD4+T cells were decreased in the other four groups (P<0.05). The sequence of CD4+T cells, from high to low, was the control group, the immunodeficiency group, the immune clearance group, the compensated cirrhosis group and the de-compensated cirrhosis group. CD4+/CD8+T cell and NK cell were lower , but CD8+T cell and B cell were higher in immune clearance group than that of the control group (P < 0.05). Patients in immunodeficiency group had lower ratio of CD4+/CD8+T cell and higher CD8+T cell than those in the control group (P < 0.05). In all the groups, patients with de-compensated cirrhosis showed highest ratio of CD4+ to CD8+ T cells and B cells, but lowest CD3+T, CD8+ T and NK cells (P < 0.05). Conclusions Results suggests immune dysfunction exists in patients with chronic HBV infection. It has potential clinical value in understanding patients′ immune states and progression of disease by detecting peripheral blood lymphocyte subsets and NK cells.
