The cloning and expression of nucleoproteins (NP) of Ebola virus
10.7644/j.issn.1674-9960.2014.09.001
- VernacularTitle:埃博拉病毒NP抗原表位区段的克隆和表达
- Author:
Xiaodan WANG
;
Pengfei LI
;
Xiaoyan FENG
;
Cuixia ZHU
;
Haiping QUE
;
Xuhui ZHANG
;
Zhiqiang LIU
;
Cuimi DUAN
;
Bingshui XIU
;
Heqiu ZHANG
- Publication Type:Journal Article
- Keywords:
Ebola virus;
nucleoprotein;
epitope;
expression
- From:Military Medical Sciences
2014;(9):659-662
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide the candidate antigens for immunological diagnosis by analyzing the expression of nu -cleoprotein ( NP) of Ebola virus. Methods BioSun software was used to predict the NP epitopes. The bridging-PCR was used to synthesize the NP gene. The pBVIL1 vector was used to clone and express the NP gene. Results The 360-739 aa of NP was confirmed to be the dominant antigen by BioSun software. The recombinant NP dominant antigen was expressed in E.coli with molecular weight of 58 ×103.The specificity of ELISA based on recombinant NP was 99.24% (130/131) in negative samples. Conclusions The dominant NP antigen can be potentially used for developing Ebola virus diagnostic reagent.
