Combination of duplex PCR technology with capillary electrophoresis is an alterna-tive method to validate the result of transcriptome sequencing
10.7644/j.issn.1674-9960.2014.09.017
- VernacularTitle:一种基于双重PCR结合毛细管电泳验证转录组测序结果的新方法
- Author:
Jianming YI
;
Yan GAO
;
Zhihui LI
;
Yanchun ZHANG
;
Wubin QU
;
Chenggang ZHANG
- Publication Type:Journal Article
- Keywords:
transcriptome sequencing;
duplex PCR;
capillary electrophoresis;
Q-PCR
- From:
Military Medical Sciences
2014;(9):724-728
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method to validate the result of transcriptome sequencing using duplex PCR tech -nology combined with capillary electrophoresis .Methods According to a previous study on transcriptome sequencing , eight differentially expressed genes were chosen as target genes for examination .The mRNA expression level of these genes was detected using duplex PCR combined with agarose gel electrophoresis , duplex PCR combined with capillary electropho-resis and Q-PCR, respectively.Then, the verification efficiency of each method was evaluated carefully .Results The ver-ification efficiency of duplex PCR combined with agarose gel electrophoresis was 50%, while that of duplex PCR combined with capillary electrophoresis and Q-PCR was both 100%.Conclusion Combination of duplex PCR technology with capil-lary electrophoresis can be used as an alternative method to validate the results of transcriptome sequencing .
