An up-converting phosphor technology based lateral flow assay for rapid detection of Listeria monocytogenes

VernacularTitle:基于上转发光免疫层析技术快速检测单核细胞增生李斯特菌
Author: Xu LIU ; Chunfeng LI ; Xiaoying WANG ; Na FENG ; Ruifu YANG ; Chengbin WANG ; Lei ZHOU
Publication Type:Journal Article
Keywords: up-converting phosphor nanoparticles; up-converting phosphor technology based lateral flow assay; Listeria monocytogenes
From: Military Medical Sciences 2014;(9):729-732
CountryChina
Language:Chinese
Abstract: Objective To develop an up-converting phosphor technology-based lateral-flow ( UPT-LF) assay for rapid detection of Listeria monocytogenes, named LM -UPT-LF.Methods Monoclonal antibodies against p 60, which was the specific virulence factor of L.monocytogenes,were prepared and covalently conjugated with up-converting phosphor nanopar-ticles (UCP-NPs) as bio-label.Then, LM-UPT-LF was established with double-antibody sandwich mode-based LF assay. Detection performance , including sensitivity and specificity , was evaluated .Results The samples with absolute contamina-ted amount of L.monocytogenes cells <10, 10-99, and 100-1000 cfu could be significantly detected as positive after in-cubation at 20 h, 18 h, and 16 h, respectively.Other 13 kinds of food-borne pathogens with concentration of 109 cfu/ml did not caused any non-specific reaction .Conclusion The established LM-UPT-LF assay could detect L.monocytogenes with high sensitivity , specificity and simplicity and provides an alternative method for food safety control .