The Study of Signaling Pathways in MCP-1 Over-Expression/Interference of HUVECs
10.3969/j.issn.0253-9896.2014.11.001
- VernacularTitle:MCP-1转染人脐静脉内皮细胞过表达/沉默后相关信号通路研究
- Author:
En SONG
;
Guangdi LI
;
Rudan ZHOU
;
Xueling ZHAO
- Publication Type:Journal Article
- Keywords:
monocytes;
chemotactic factors;
endothelium,vascular;
umbilical veins;
signal transduction;
venous thrombosis;
monocyte chemoattractant protein-1;
human umbilical vein endothelial cells
- From:
Tianjin Medical Journal
2014;(11):1057-1061
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between the signaling pathways of MCP-1-pCDH-GFP-trans?fected cells and deep venous thrombosis (DVT). Methods The cultured human umbilical vein endothelial cells (HUVECs) were tested by immunofluorescence and co-immunoprecipitation methods. The constructed MCP-1 over-expression/interfer?ence vector, and the change of transcription profile were detected by microarray assay and biological information technology analysis. Results MCP-1 over-expression/interference vector MCP-1-pCDH-GFP/MCP-1-LMP shRNAmir1 was con?structed and HUVECs were transfected. According to the microarray analysis we found that there were 18 down-expressed signaling pathways and 7 up-expressed signaling pathways in MCP-1-pCDH-GFP-transfected cells. There were 60 down-expressed signaling pathways and 15 up-expressed signaling pathways in the MCP-1-LMP shRNAmir1 transfected cells. Conclusion Signaling pathways of MCP-1 plays an important role in DVT formation,which may provide us a new way to study molecular mechanism of DVT.
