Study on NIH3T3 cells infected by retrovirus encoding EGFP fusion of human ribonuclease inhibitor protein
10.3969/J.ISSN.1672-8270.2014.10.007
- VernacularTitle:绿色荧光蛋白融合人核糖核酸酶抑制因子病毒感染NIH3T3细胞的研究
- Author:
Kun LI
;
Ning DING
- Publication Type:Journal Article
- Keywords:
Human ribonuclease inhibitor;
Infection;
Enhanced green fluorescent protein
- From:
China Medical Equipment
2014;(10):20-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the expression of egfp-hri fusion on NIH3T3 cells which is carried by recombinant retrovirus. Methods:The egfp-hri fusion gene was integrated intoNIH3T3 cells by the infection of recombinant viral supernatant from the G418 resistent PA317 cells transfected stably with the plasmid of pLNCX-EGFP-C1-hri. The expression effect of egfp-hri fusion was visualized under the fluorescent microscope, while the expression levels of egfp-hri fusion protein by Western blot. Results:The observation from fluorescent microscope showed the green fluorescent was obviously observed in the cytoplasm , western-blotting showed the expression level of egfp-hri fusion gene increased by 83%and 81%, as compared with those uninfected cells and in the cells infected with empty vector, respectively. Conclusion:The infected NIH3T3 cells can express egfp-hri fusion protein successfully.
