Influence of paclitaxel on the correlation factors in serum and tissue of patients with metrocarcinoma
10.3760/cma.j.issn.1008-6706.2014.21.018
- VernacularTitle:紫杉醇对子宫癌患者血清及组织相关因子的影响
- Author:
Qiong LI
- Publication Type:Journal Article
- Keywords:
Paclitaxel;
Uterine Neoplasms;
Hypoxia-Inducible Factor 1,alpha Subunit;
Vascular Endothelial Growth Factors
- From:
Chinese Journal of Primary Medicine and Pharmacy
2014;(21):3246-3248
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the level of hypoxia-inducible factor-1α( HIF-1α) and vascular endotheli-al growth factor( VEGF) in serum and tissue of patients with metrocarcinoma.Methods 48 patients diagnosed with cervical cancer were analyzed retrospectively.Patients were treated with paclitaxel combination chemotherapy,HIF-1αand VEGF levels in serum of patients were detected by ELISA method before and after the treatment.HIF-1αand VEGF levels in cervical tissue of patients were observed by immunehistochemical staining methods.Western blot method was used to detect the change of HIF-1αand VEGF expression levels in cervical tissues of patients.And the levels of HIF-1αand VEGF in patients with lymphatic metastasis and lymph node metastases were compared. Results Lymph node metastasis did not occur in patients with uterine cancer serum HIF-1αand VEGF were (16.71 ±34.75)ng/L,(255.77 ±87.99)ng/L,respectively,and those after treatment were (98.33 ±32.17)ng/L, (153.67 ±65.45)ng/L,the differences were statistically significant(t=9.416,5.001,P=0.003,0.007) before and after treatment;The serum of patients with lymph node metastasis of uterine cancer in HIF-1αand VEGF increased, and cervical cancer patients after treatment,cervical tissue HIF-1αand VEGF;The metastasis were statistically signif-icant differences occurred(t=3.055,16.196,P=0.038,0.000);HIF-1αand VEGF decreased after treatment,the expression levels were significantly decreased compared with before treatment ( t=5.899,P=0.004;t=9.649,P=0.001);patients with lymph node metastasis after treatment,the expression levels of HIF-1αdecreased compared with before treatmen ( t =5.233, P =0.004), but there was no significant changes in the expression of VEGF. Conclusion The mechanism of taxol anti-uterine cancer may be related with inhibition the expression of HIF-1αand VEGF protein,and thereby inhibiting tumor angiogenesis and cancer metastasis.
