Analysis of genotype spectrum in condyloma acuminate tissues HPV infection of female anus and anal canal
10.3969/j.issn.1673-4130.2015.01.012
- VernacularTitle:女性肛门及肛管尖锐湿疣组织 HPV 感染基因型谱的分析
- Author:
Jinhao ZHANG
;
Weimin CAI
;
Jianxiang GENG
;
Lin XIA
;
Hongjing WANG
;
Kunlan WU
;
Xin SHAO
;
Jing MEI
;
Xue ZHAO
- Publication Type:Journal Article
- Keywords:
anus and anal canal;
condyloma acuminata;
human papillomavirus;
genotype;
gene-chips technique
- From:
International Journal of Laboratory Medicine
2015;(1):30-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the genotypes of human papillomavirus (HPV)infection in female anus and anal canal condylo-ma acuminata(CA)tissues and their clinical significance.Methods 23 kinds of HPV-DNA were extracted from the paraffin-embed-ded anus and anal canal tissue samples in 140 cases of female CA and detected by using PCR combined with the gene-chips tech-nique.Furthermore the related clinical pathological data of the patients were analyzed.Results Among 140 female anus and anal ca-nal CA tissue samples,103 cases were HPV positive and the total HPV infection rate was 73.57%(103/140).Among them,68 ca-ses were single type HPV infection,the positive detection rate was 48.57%(68/140)and 35 cases were multiple types HPV infec-tion,the positive detection rate was 25.00% (35/140).In single type HPV infection,34 cases were HPV11 and the positive detec-tion rate was 24.29% (34/140),HPV11 was the main infection type,followed by HPV 6 in 27 cases,its positive detection rate was 19.29%(27/140).In the multiple types HPV infection,13 cases were HPV 6 + 11,accounting for 37.14% (13/35 )of multiple types infection,followed by HPV11 +18 in 3 cases and HPV 6+11+16 in 3 cases,each accounting for 8.57%(3/35)of the multi-ple types infection.Conclusion HPV 6,11 ,6+11,11 +18 and 6+11+16 are the main infection genotypes in female anus and anal canal CA.PCR combined with the gene-chips technique is a diagnostic method more suitable for clinical development of HPV geno-typing detection,which has high sensitivity and good specificity and is especially suitable for the molecular epidemiology study of HPV infection.
