Influence on the path of signal transduction of ERK by acupuncture in rats of experimental acute intercere-bral hemorrhage
10.3760/cma.j.issn.1008-6706.2014.22.002
- VernacularTitle:针刺对脑出血大鼠细胞外信号调节蛋白激酶通路影响的研究
- Author:
Fang LIU
;
Minghui LI
;
Jian LI
;
Xiaowei SUN
- Publication Type:Journal Article
- Keywords:
Acupuncture;
Intracerebral Hemorrhage;
Extracellular signal-regulated protein kinases;
Wisten
- From:
Chinese Journal of Primary Medicine and Pharmacy
2014;(22):3364-3366
- CountryChina
- Language:Chinese
-
Abstract:
Objective To confirm the influence on the path of signal transduction of ERK by BaiHui-QuBin Scalp-acupuncture in rats of experimental acute intercerebral hemorrhage.Methods 192 healthy Wistar male rats were selected by examination.The rats were assigned into 4 groups randomly:matched blank group,model group,pin-prick group,excitomotor group.The latter three groups contained 60 rats in each;another 12 rats were assigned as the matched blank group.All rats were made to be the intercerebral hemorrage model.The pinprick group was acupunc-tured on the BaihuipenetratingQubin.We observed the change surrounding the hamatoma of the proteinum expression of p-ERK of each group in deferent time phase point with the method of Western blot.Results All of the rats after being made model appeared the phenomenon of the expression of p-ERK of rat's brain tissue surrounding the hematoma and increased to the peak in 7 days.1 d, 2 d, 3 d, 7 d, compare with the model group [(0.10 ±0.04),(0.17 ±0.02),(0.09 ±0.05),(0.10 ±0.06)],the expressions of p-ERK of the pinprick group [(0.19 ±0.04),(0.23 ±0.07),(0.24 ±0.05),(0.25 ±0.06),q=11.45,P<0.01] and the excitomotor group's were higher obviously[(0.19 ±0.05),(0.20 ±0.05),(0.24 ±0.06),(0.25 ±0.09),(0.26 ±0.09),q=10.07, P<0.01].The pinprick group compared with the excitomotor set,the expressions of p-ERK showed no significant difference(P>0.05).Conclusion The therapy of BaiHui-QuBin Scalp-acupuncture can activate the path of signal transduction of ERK,increase the expression of the proteinum p-ERK and so on,which have the function of protecting the nerve cell.
