Association analysis of 5509-5511delCAA polymorphism in Tim-1 exon4 with rheumatoid arthritis in Han population of Yunnan province
10.3969/j.issn.1673-4130.2015.01.005
- VernacularTitle:云南汉族人群 Tim-1外显子4上5509-5511delCAA基因多态性与类风湿关节炎关联性研究
- Author:
Xianghong CAO
;
Chuanmei PENG
;
Hui GAO
;
Xiaoye FU
;
Yulin DONG
;
Yang WANG
- Publication Type:Journal Article
- Keywords:
rheumatoid arthritis;
T cell immunoglobulin domain and mucin domain protein-1;
gene polymorphism;
sus-ceptibility
- From:
International Journal of Laboratory Medicine
2015;(1):12-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the association between the polymorphism of 5509-5511delCAA in exon4 of Tim-1 gene and the susceptibility to rheumatoid arthritis(RA)in a group of Han population from Yunnan province.Methods The PCR-DNA se-quencing analysis was used to detect the polymorphism of 5509-5511delCAA in exon4 of Tim-1 gene in 196 RA Han nationality pa-tients and 190 individuals of healthy physical examination(control).The ELISA method and indirect immunofluorescence assay was adopted to detect the three related aotuantibodies of RA,including the rheumatoid factors(RF)and the anti-cyclic citrullinated pep-tide(Anti-CCP)and the anti-keratin antibodies(AKA).Results There were statistically significant differences in genotype and al-lele frequency of 5509-5511delCAA between the RA group and the control group(P <0.05);the positive rate of RF and the anti-CCP between different genotypes of 5509-5511delCAA in the RA group had no statistically significant differences(P >0.05 ),but the positive rate of AKA between different genotypes of 5509-5511delCAA in the RA group had statistically significant differences (P <0.05).Conclusion The 5509-5511delCAA site of Tim-1 exon4 has the polymorphic variation,the 5509-5511delCAA genotype maybe related with the genetic susceptibility to RA in Han population of Yunnan province,the different genotypes does not affect the expression of RF and the anti-CCP,but affects the expression of AKA.
