Clinical application of ultraviolet spectrophotometric method in detection of colchicine poisoning serum
10.3969/j.issn.1673-4130.2015.01.015
- VernacularTitle:紫外分光光度法测定秋水仙碱中毒血清的临床应用
- Author:
Junsheng LIU
;
Shuping WANG
;
Shuming DU
- Publication Type:Journal Article
- Keywords:
colchicines;
poisoning;
serum;
UV spectrophotometry
- From:
International Journal of Laboratory Medicine
2015;(1):37-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application of the ultraviolet spectrophotometry method in detecting colchicine poisoning serum to provide the basis for clinical diagnosis and treatment of colchicine poisoning.Methods 0.5 mL of serum was taken and added with the extract solution(chloroform∶methanol=95 ∶5)4.0 mL.The sufficient oscillation extraction and high-speed cen-trifugation were performed.Then the organic layer was taken into another test tube for drying by nitrogen.0.2 mL of methanol dis-solved residue was taken and blended.50 μL of the mixed solution was taken for conducting ultraviolet scanning.Results The max-imum absorption peak of serum colchicine was (351 ±1)nm,the concentration within 5.0-40 μg/mL showed linearity,the regres-sion equation wasY =0.050 2X +0.001 3,the correlation coefficient was 0.999 5,the recovery rate was 83.8% -102.8%,the rel-ative standard deviation:3.3% -4.8%.The intra-day and inter-day were 3.22%-4.74% and 3.45%-4.66%,the lowest detec-tion concentration was 1 .0 μg/mL.Conclusion This method is simple to operate,fast in analysis,accurate in the detection result, which provides a simple and accurate detection method for clinical diagnosis of colchicine poisoning.
