Study on the effects of intestinal trefoil factor on gastric mucosal epithelial cell proliferation and its signal transduction mechanism
- VernacularTitle:肠三叶因子对胃黏膜上皮细胞增殖的影响及其信号转导机制的研究
- Author:
Zhaorui SUN
;
Zhizhou YANG
;
Jinfeng LIN
;
Danbing SHAO
;
Hongmei LIU
;
Min XU
;
Wei ZHANG
;
Baodi SUN
;
Yi REN
;
Binxia SHAO
;
Baohua XU
;
Wenjie TANG
;
Shinan NIE
- Publication Type:Journal Article
- Keywords:
GES-1 cells;
Intestinal trefoil factor;
Proliferation;
Regulation;
PI3K/Akt signaling pathway
- From:
Journal of Medical Postgraduates
2014;(10):1023-1027
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of intestinal trefoil factor ( ITF) on gastric mucosal epithelial cell proliferation and its possible molecular mechanism . Methods The cultured GES-1 cells were treated with ITF in the concentration of 100 ng/mL and 500 ng/mL in vitro, and then were observed using microscope for the morphological analysis .The Cell Counting Kit-8 ( CCK-8) was used to detect the proliferation activity of GES-1.The cultured GES-1 cells were treated with 100 ng/mL ITF and the specific inhibitor of PI3K/Akt signaling pathway LY294002 (15μmol/L) in vitro, and then were observed using microscope for the morphological analysis . The proliferation activity of treated GES-1cells was detected using CCK-8 and the expressions of p-Akt and Akt of PI3K/Akt signaling pathway were determined by Western blot . Results Compared with the control group , the proliferation activity of GES-1 cells in-creased after being treated with ITF and the higher concentration of ITF induced the higher proliferation activity .LY294002 inhibited the increased proliferation activity of GES-1induced by ITF.The data of Western blot indicated that ITF induced the expression of p -Akt and activated the P3IK/Akt signaling pathway to modulate the proliferation activity of GES -1 cells.However, LY294002 inhibited the PI3K/Akt signaling pathway and then decreased the proliferation activity of GES -1 cells. Conclusion ITF could promote the proliferation ac-tivity of GES-1 cells by activating PI3K/Akt signaling pathway .
