Cell factor analysis of SD rat BMSC-liver cells co-culture supernatant
10.3969/j.issn.1671-8348.2014.28.023
- VernacularTitle:SD大鼠骨髓间充质干细胞-肝细胞共培养上清液细胞因子分析
- Author:
Na WANG
- Publication Type:Journal Article
- Keywords:
stem cells;
bone marconi;
hepatocytes;
coculture techniques;
cytokines
- From:
Chongqing Medicine
2014;(28):3766-3768
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect supernatant of SD rat bone marrow mesenchymal stem cells(BMSC)and liver cells,and explore the role of cytokines,and provide a theoretical basis for the biological artificial liver and clinical treatment of liver failure.Methods BMSCs and primary hepatocytes of SD rat were isolated.BMSCs with its third generation and liver cells by the ratio of 1∶10 were co-cultured.Hepatocytes and BMSC were treated as control group.Observation the cells′survival and morphology and analysis of cytokine after 48 h were made by RayBiotech.Results BMSC-hepatocyte in the co-culture group grow and proliferated rapidly,and hepatocyte could survive up to 14 days;while hepatocyte cultured alone,it grow slowly and survived only 9 days.Expression of BM-SC-liver cells supernatant changed obviously:interleukin 1,interleukin 6,interleukin 10 were higher than those in the control group,above 2 times,which showed significant difference (P<0.01).Conclusion Co-culture of BMSC-liver cell could induce BM-SC secrete paracrine and autocrine:IL-1 and IL-6 and IL-10,which could promote the growth of hepatocyte and extend the liver cell survival time.In-vitro BMSC-liver cells could provide biological artificial liver cell sources,as well as provide theory basis for using cytokine treatment for liver failure.
