Influence of Electro-acupuncture on E-Selectin and Resistin Protein Expression in Hippocampus of Rats with Chronic Stress Depression
10.3969/j.issn.1005-5304.2014.11.017
- VernacularTitle:电针对抑郁大鼠海马E-选择素和抵抗素蛋白表达的影响
- Author:
Cheng HU
;
Danmei ZHANG
;
Wuye BAO
;
Yingzhou SONG
;
Rongxing SHI
;
Ya TU
;
Jihong WU
- Publication Type:Journal Article
- Keywords:
electro-acupuncture;
depression;
chronic stress;
hippocampus;
vascular endothelial cell;
E-selectin;
resistin;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(11):56-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of electro-acupuncture on the E-selectin and resistin protein expression in the hippocampus of rats of chronic stress depression;To explore the role and mechanism of electro-acupuncture in the micro-environment of brain with depression. Methods 40 Fourty rats were randomly divided into four groups:blank group, model group, model+EA group, and model+fluoxetine group by using chronic stress combined with solitary raising methods, 10 rats in each group. Electro-acupuncture intervention was used 1 hour before the modeling. Electro-acupuncture was given at points Baihui (Du 20) and Yintang (Extra) for twenty minutes (2 Hz, 0.6 mA) every day. Fluoxetine was given at the volume of 5 mL/kg, dosage of 10 mg/kg. E-selectin and resistin protein expression in hippocampus were determined by biotin label-based antibody array. Results Compared with the blank group, the protein expression of E-selectin and resistin in the model group increased (fold change=1.23, 1.22). Compared with the model group, E-selectin and resistin expression decreased (fold change=0.65, 0.62;fold change=0.76, 0.65). Conclusion Electro-acupuncture intervention could down-regulate E-selectin and resistin expression in the hippocampus of chronic stress depression model.
