Effects of Tangshenkang on MMP-9 and TIMP-1 of Human Renal Tubular Epithelial Cell HK-2 Induced by High Glucose
10.3969/j.issn.1005-5304.2014.10.017
- VernacularTitle:糖肾康对高糖诱导人肾小管上皮细胞分泌基质金属蛋白酶-9及其抑制剂-1的影响
- Author:
Qingchun CHAI
;
Lixia YANG
;
Jianjun XUE
;
Tao CHENG
;
Dinghua ZHANG
;
Tonghua LIU
- Publication Type:Journal Article
- Keywords:
Tangshenkang;
high glucose;
human renal tubular epithelial cell;
MMP-9;
TIMP-1
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(10):58-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Tangshenkang in the prevention and treatment of diabetic nephropathy. Methods HK-2 cells were cultured in vitro and divided into control group, high glucose group (30 mmol/L D-glucose), control group (30 mmol/L D-glucose+10% animal serum), and Tangshenkang drug-containing serum therapy groups (30 mmol/L D-glucose+5%low concentration Tangshenkang, 30 mmol/L D-glucose+10%middle concentration Tangshenkang, 30 mmol/L D-glucose+20% high concentration Tangshenkang). After 24 h and 48 h treatment, MMP-9 and TIMP-1 in cell cultural supernatant were observed by ELISA. Results MMP-9 of HK-2 cultured with high glucose was much decreased and TIMP-1 increased significantly than the control group, with statistical significance (P<0.05). TIMP-1 significantly decreased and MMP-9 increased in HK-2 cultured with high glucose plus Tangshenkang compared with those only induced by high glucose, with statistical significance (P<0.05). Conclusion Tangshenkang could regulate the secretion of fibrosis cell factor of HK-2 cell induced by high glucose, which may be one of the mechanisms in its treatment of diabetic nephropathy.
