Study on Quality Standard for Biqing Suppository
10.3969/j.issn.1005-5304.2014.10.021
- VernacularTitle:萆清栓质量标准研究
- Author:
Tao SHEN
;
Haining LIANG
;
Yingyan BI
- Publication Type:Journal Article
- Keywords:
Biqing Suppository;
berberine hydrochloride;
TLC;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(10):71-73
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quality standard for Biqing Suppository. Methods Rhizoma Dioscoreae Septemlobae, Rhizoma Acori Tatarinowii, Radix Et Rhizoma Salviae Miltiorrhizae and Cortex Phellodendri Chinensis were qualitatively identified by TLC method. The content of berberine hydrochloride in Biqing Suppository was determined by HPLC method. The chromatographic column was Waters C18 (250 mm×4.6 mm, 5 μm);the mobile phase was acetonitrile∶water (0.2%phosphoric acid-0.02%three triethylamine)=25∶75;the flow rate was 1 mL/min;the detection wavelength was 265 nm. Results The spots of qualitative identification method were clear without interference. Berberine hydrochloride had good linear relationship in the range of 0.235-9.40 μg;the regression equation was Y=40 033 998.176 3X-85 021.2, r=0.999 7;the average recovery rate was 99.16%, RSD=3.45%. Conclusion The method is simple, flexible and reproducible, which can be used as the quality control standard for Biqing Suppository.
