Preparation, Purification and Property of Polysaccharides in Shuanqian Fungal Substance
10.3969/j.issn.1005-5304.2014.10.024
- VernacularTitle:栓钱菌质多糖的制备、纯化及性质研究
- Author:
Xuexiang LIU
;
Jianwei CHEN
- Publication Type:Journal Article
- Keywords:
Strychnos nux-vomica;
Trametes cinnabarina;
fungal substance;
polysaccharide
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(10):80-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare and purify the polysaccharides in Shuanqian fungal substance (FS);To study its monosaccharide composition and property. Methods Strychnos nux-vomica was under a thirty-day solid fermentation through Trametes cinnabarina. Then crude polysaccharide was got after the process of drying, smashing, water boiling, condensing, decoloring, removing protein and ethanol precipitation. The content of polysaccharides in Shuanqian FS was determined by phenol-sulfuric acid method. Dialysis and gel chromatography method was used to purify the crude polysaccharide. The purified composition was analyzed by acid hydrolysis and thin layer chromatography. Results The content of crude polysaccharide was 47.68%and yield was 10.52%. It showed that polysaccharide in Shuanqian FS contained glucose and galactose, but no nucleic acid and protein. Conclusion Polysaccharide in Shuanqian FS was an offwhite powdery heteropolysaccharide, which contained glucose and galactose.
