Content Determination Method of α-asarone in Dianxianqing Granules
10.3969/j.issn.1005-5304.2014.10.025
- VernacularTitle:癫痫清颗粒中α-细辛醚含量测定方法研究
- Author:
Jinghan ZHANG
;
Guanghan WANG
;
Guixin ZOU
- Publication Type:Journal Article
- Keywords:
Dianxianqing Granules;
α-asarone;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(10):83-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the content determination method ofα-asarone in Dianxianqing Granules. Methods Kromasil C18 column (250 mm×4.6 mm, 5 μm) was used, with methanol and water as mobile phase of gradient elution. The flow rate was 1.0 mL/min, and the detection wavelength was 257 nm. The column temperature was 25 ℃. Results The sample solution was prepared by reflux extracted with 70% ethanol for 20 min. α-asarone had a good linearity in the ranges of 1.24×10-3-7.44×10-3μg (r=0.999 5). The method was quite good in precision, stability and repeatability, and the average recovery ofα-asarone was 98.14%, with corresponding RSD of 2.5%. Conclusion The experiment provides an accurate and fast analytical method for α-asarone determination in compound preparation of traditional Chinese medicine.
