Different Nrf2 expressions in glioblastoma cell lines and glioma stem cells from xenografts
10.3969/j.issn.1006-5725.2014.17.005
- VernacularTitle:Nrf2在胶质瘤干细胞和胶质母细胞瘤细胞系中的差异表达
- Author:
Jianhong ZHU
;
Handong WANG
;
Youwu FAN
;
Qing SUN
;
Xiangjun JI
;
Huandong LIU
;
Mengliang ZHOU
- Publication Type:Journal Article
- Keywords:
Glioma stem cell;
Nrf2;
Glioblastoma
- From:
The Journal of Practical Medicine
2014;(17):2709-2711
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare different Nrf2 expressions in glioblastoma cell lines and glioma stem cells (GSCs) from xenografts and to study the concentration of Nrf2 in nuclear. Methods GSCs were analyzed by immunofluorescence and different expressions of Nrf2 in glioblastoma cell lines and GSCs from xenografts were detected with real-time RCR and Western. Results GSCs were successfully isolated from xenografts of U251 and U87 cell lines. The percentage of tumor stem cells in total cells was 1.24%, and that was 1.63% in xenografts. Immunofluorescence indicated that Nrf2 was overexpressed in GSCs as compared with that in glioblastoma cell lines. Conclusion Nrf2 may be a potential biomarker and rational therapeutic target for GSCs.
