Effects of simvastatin on lung tissue angiogenesis and the gene expression of VEGF and PF4 of rats with pulmonary fibrosis
10.3969/j.issn.1006-5725.2014.17.006
- VernacularTitle:辛伐他汀对肺纤维化鼠肺组织血管新生及 VEGF 和PF4基因表达的影响
- Author:
Wei CHEN
;
Shao OUYANG
;
Zhenhua HE
;
Xiaowu TAN
;
Xiufeng ZHANG
;
Jianfeng XIE
- Publication Type:Journal Article
- Keywords:
Pulmonary fibrosis;
Angiogenesis;
Simvastatin;
VEGF;
PF4
- From:
The Journal of Practical Medicine
2014;(17):2711-2714
- CountryChina
- Language:Chinese
-
Abstract:
Objective The study was designed to observe influence of simvastatin on lung tissue angiogenesis and the gene expression of vascular endothelial growth factor(VEGF) and platelet factor 4(PF4) of rats with bleomycin (BLM)-induced pulmonary fibrosis. Methods Ninety-six healthy male SD rats were divided into four groups by random number table, including normal control group (A), bleomycin group (B), prednisone acetate treatment group (C) and simvastatin treatment group (D). Lung tissue of rats in each group was detected as specimens. HYP was detected by digestion method. Angiogenesis, VEGF and PF4 protein expression were determined by immunohistochemical method (SP). Expression of VEGF and PF4 mRNA were respectively detected by RT-PCR assay. Results (1)HYP content of group C, D was lower than the group B, which was statistical significance (P <0.01). (2)MVD and the expression of VEGF in group B, C and D was higher than that in group A. PF4 expression of group B, C and D were lower than that of group A (P < 0.01). MVD and the expression of VEGF of group D were lower than those of group B, the expression of PF4 of group D was higher than that in group B (P < 0.05). Conclusion Mechanism of simvastatin on pulmonary fibrosis may be related to regulate the expression of VEGF and PF4 in lung tissue, inhibit pathological angiogenesis.
