Effects of Different Processing Methods on the Contents of Oleanolic Acid and Ursolic Acid in Chaenomeles Medicinal Materials
10.3969/j.issn.1005-5304.2014.11.023
- VernacularTitle:不同产地加工法对宣木瓜药材中齐墩果酸和熊果酸含量的影响
- Author:
Xiaoge WANG
;
Quanjiang LONG
;
Zhou ZHOU
;
Yue ZHAO
;
Tiantian ZHOU
- Publication Type:Journal Article
- Keywords:
chaenomeles specios;
processing method in producing area;
HPLC;
oleanolic acid;
ursolic acid
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(11):75-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of different processing methods on the contents of oleanolic acid and ursolic acid in chaenomeles medicinal materials. Methods The contents of oleanolic acid and ursolic acid in samples processed by different processing methods in producing area were determined by HPLC with Agilent HC-C18 (2) chromatographic column (4.6 mm×250 mm, 5 μm), mobile phase of methanol-water-acetic acid-triethylamine (89∶11∶0.1∶0.05), detection wavelength of 210 nm, column temperature at 25 ℃, the flow rate of 1.0 mL/min. Results The total contents of oleanolic acid and ursolic acid in the samples prepared through 4 different processing methods were 0.86%, 0.93%, 1.29%, and 1.47%, respectively, showing that different processing methods in producing area had a significant effect on contents of oleanolic acid and ursolic acid in chaenomeles medicinal materials. Conclusion This study provides a basis for processing methods of chaenomeles medicinal materials in producing area.
