Simultaneous Content Determination of Alantolactone and Isoalantolactone of Radix Inulae in Sanwei Ganlusan by HPLC
10.3969/j.issn.1005-5304.2014.11.026
- VernacularTitle:HPLC同时测定三味甘露散中土木香内酯和异土木香内酯含量
- Author:
Xiujuan ZHANG
;
Yonghui DING
;
Lin NI
;
Xi YANG
- Publication Type:Journal Article
- Keywords:
Sanwei Ganlusan;
HPLC;
alantolactone;
isoalantolactone
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(11):85-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method to simultaneously determine contents of alantolactone and isoalantolactone in Sanwei Ganlusan. Methods Contents of alantolactone and isoalantolactone was determined by HPLC was used with PDAD detector;the column was CAPCELL PAK MG C18 (4.6 mm×250 mm, 5μm);the mobile phase consisted of acetonitrile-0.4%phosphoric (58∶42);the flow rate was 1.0 mL/min;the detection wavelength was set at 225 nm;the column temperature was maintained at 30 ℃. Results The linear range of alantolactone was 0.083-0.517 μg (r=0.999 9), and isoalantolactone was 0.108-0.672 μg (r=0.999 9). The mean recovery of alantolactone was 97.95%, RSD=1.31%. The mean recovery of isoalantolactone was 97.69%, RSD=1.24%. Conclusion The method is accurate and simple in operation, which can be used to simultaneously determine contents of alantolactone and isoalantolactone in Sanwei Ganlusan.
