TLC Identification and HPLC Fingerprint of Paeoniae Radix Alba
10.3969/j.issn.1005-5304.2014.11.029
- VernacularTitle:白芍药材薄层色谱鉴别和高效液相色谱特征图谱研究
- Author:
Shengshi JIANG
;
Xiaoqing PENG
- Publication Type:Journal Article
- Keywords:
Paeoniae Radix Alba;
TLC;
HPLC;
fingerprint
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(11):95-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study TLC and HPLC fingerprint of Paeoniae Radix Alba;To provide reliable methods for its quality control and evaluation. Methods TLC was performed to detect paeoniflorin with CHCl3-EtOAc-methanol-formic acid (40∶5∶10∶0.2) as the developing solvent system. The HPLC chromatographic separation was performed on Kromasil C18 column (250 mm× 4.6 mm, 5μm, 12 nm). The mobile phase was a mixture of acetonitrile-0.05%phosphoric acid in gradient elution with flow rate of 1 mL/min. The detection wavelength was set at 254 nm, and the column temperature was 35 ℃. Results The TLC spots were clear. Fifteen common peaks including paeoniflorin (the ninth peak) in the HPLC profile were found in ten batches of samples of Paeoniae Radix Alba in different growing areas, the fingerprints of samples from Anhui Province and Zhejiang Province showed little difference. Conclusion Fingerprint of Paeoniae Radix Alba showed unique features, and can be used to control its quality.
